Contact an Account ManagerRetention behaviour of bile acids and ion pair chromatography on bonded phases
Summary
Type: HPLC
Application #: 100010
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: UV
Conditions: eluent: (A) acetonitrile - phosphate buffer (30:70), pH 7.5, containing 5*10-3 M tetrabutylammonium hydrogen sufphate (TBA), (B) acetonitrile - phosphate buffer (65:35), pH 7.5, containing 5*10-3 M TBA;
flow rate 1 ml/min
Authors: Lu, D.S. et al.
Source: J. Chromatogr. 268 (1983) 1–18
Details
Legend: Chromatogram showing separation of bile acids with one, two and three
substituents in IPC using gradient elution
7,12-OC = 7,12-dioxocholic acid,
7- and 12-OC = 7- and 12-oxocholic acids,
TOCA =